Two-colour microarray experiments

Annotare currently only supports one type of two-colour microarray workflow (see below). For other workflows, please continue to use Annotare to construct the submission (e.g. experiment background, protocols, sample description, assigning files to labeled extracts).

When you get to the stage where you have validation errors about "[Assign Files] The assignment between files and samples contains errors.", please write us using the "Contact Us" button from within your submission form and a curator will assist. We will be supporting more workflows in the next few months.

The supported workflow has a 2:2:1:1 relationship between samples, labelled extracts, hybridisation assays and data files:

For this workflow, you need to tell us which label (fluorescent dye) is assigned to each extract, e.g. Cy3 or Cy5. By default Annotare uses your sample names as your extract names.

The experiment above would be represented like this through the different pages of Annotare forms:

For more help on how to assign files for two-colour microarray experiments, see two-colour section of the Assign Files page.